Journal: Cells

Article Title: Probiotics in the Prevention of the Calcium Oxalate Urolithiasis

doi: 10.3390/cells11020284

Figure Lengend Snippet: A summary of previous studies on the use of Lactobacillus spp. for the prevention of urolithiasis.

Article Snippet: Turroni et al. [ ] , In vitro , The oxalate-degrading activities of 14 Bifidobacterium strains ( B. adolescentis ATCC 15703, B. animalis subsp. Lactis DSM 10140, BA30, Bb12, BI07, and L15, B. bifidum S16, B. breve ATCC 15700 and BBSF, B. catenulatum B665, B. longum biotype longum S123, ATCC 15707, and W11, and B. longum biotype suis ATCC 27533), cultured in oxalate-rich media, was measured by a capillary electrophoresis technique. The identification of the oxc gene was performed with PCR , Only the five B. animalis subsp. Lactis strains (DSM 10140, BA30, Bb12, BI07, and L15) showed oxalate-degrading activity with 100% of oxalate consumption after 5 days of incubation, whereas no degrading activity was exhibited by all the other bifidobacterial strains tested..

Techniques: In Vitro, Bacteria, Activity Assay, Cell Culture, Electrophoresis, Incubation, Expressing, Isolation, Extraction, In Vivo, Clinical Proteomics, Concentration Assay, Animal Model, Microscopy, Control

Journal: Cells

Article Title: Probiotics in the Prevention of the Calcium Oxalate Urolithiasis

doi: 10.3390/cells11020284

Figure Lengend Snippet: Characteristics of Bifidobacterium spp.

Article Snippet: Turroni et al. [ ] , In vitro , The oxalate-degrading activities of 14 Bifidobacterium strains ( B. adolescentis ATCC 15703, B. animalis subsp. Lactis DSM 10140, BA30, Bb12, BI07, and L15, B. bifidum S16, B. breve ATCC 15700 and BBSF, B. catenulatum B665, B. longum biotype longum S123, ATCC 15707, and W11, and B. longum biotype suis ATCC 27533), cultured in oxalate-rich media, was measured by a capillary electrophoresis technique. The identification of the oxc gene was performed with PCR , Only the five B. animalis subsp. Lactis strains (DSM 10140, BA30, Bb12, BI07, and L15) showed oxalate-degrading activity with 100% of oxalate consumption after 5 days of incubation, whereas no degrading activity was exhibited by all the other bifidobacterial strains tested..

Techniques: Staining

Journal: Cells

Article Title: Probiotics in the Prevention of the Calcium Oxalate Urolithiasis

doi: 10.3390/cells11020284

Figure Lengend Snippet: A summary of previous studies on the use of Bifdobacterum spp. for the prevention of urolithiasis.

Article Snippet: Turroni et al. [ ] , In vitro , The oxalate-degrading activities of 14 Bifidobacterium strains ( B. adolescentis ATCC 15703, B. animalis subsp. Lactis DSM 10140, BA30, Bb12, BI07, and L15, B. bifidum S16, B. breve ATCC 15700 and BBSF, B. catenulatum B665, B. longum biotype longum S123, ATCC 15707, and W11, and B. longum biotype suis ATCC 27533), cultured in oxalate-rich media, was measured by a capillary electrophoresis technique. The identification of the oxc gene was performed with PCR , Only the five B. animalis subsp. Lactis strains (DSM 10140, BA30, Bb12, BI07, and L15) showed oxalate-degrading activity with 100% of oxalate consumption after 5 days of incubation, whereas no degrading activity was exhibited by all the other bifidobacterial strains tested..

Techniques: In Vitro, Bacteria, Western Blot, Activity Assay, Electrophoresis, Extraction, Probiotics, Cell Culture, Knock-Out

Journal: Cells

Article Title: Probiotics in the Prevention of the Calcium Oxalate Urolithiasis

doi: 10.3390/cells11020284

Figure Lengend Snippet: Characteristics of O. formigenes , Lactobacillus and Bifidobacterium in the prevention of urolithiasis.

Article Snippet: Turroni et al. [ ] , In vitro , The oxalate-degrading activities of 14 Bifidobacterium strains ( B. adolescentis ATCC 15703, B. animalis subsp. Lactis DSM 10140, BA30, Bb12, BI07, and L15, B. bifidum S16, B. breve ATCC 15700 and BBSF, B. catenulatum B665, B. longum biotype longum S123, ATCC 15707, and W11, and B. longum biotype suis ATCC 27533), cultured in oxalate-rich media, was measured by a capillary electrophoresis technique. The identification of the oxc gene was performed with PCR , Only the five B. animalis subsp. Lactis strains (DSM 10140, BA30, Bb12, BI07, and L15) showed oxalate-degrading activity with 100% of oxalate consumption after 5 days of incubation, whereas no degrading activity was exhibited by all the other bifidobacterial strains tested..

Techniques: Bacteria, Membrane, Probiotics, Activity Assay, Lyophilization, Formulation, Capsules, Clinical Proteomics